FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49808547
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG154000.012.45145745
GGCATAG173400.010.73185435
GCGTTCG32350.010.710628515
ATAGGGG177150.010.70861138
CCGCGTT33750.010.6661313
CGCGTTC33050.010.6199214
GTTGTCG19200.010.54650326
GAGCTTA180450.010.45173644
GGGAGGT283700.010.4034223
GGAGGTC286450.010.1620654
CGTTCGC34750.010.09960316
TATCCTA106150.09.9603445
TAGGCTA98450.09.78100610
TGGCATA194200.09.75645634
TGCCCGT119150.09.74668544
CATAGGG197350.09.58966437
CCTAGGC102150.09.5794378
ACTCTCC301250.09.44753511
GTCACTC310600.09.3862618
ATCCTAG127400.09.3581386
Produced by FastQC (version 0.11.5)