FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55608209
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1026470.184589652941349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT205550.023.47777645
AGACCGC244950.019.5995164
CAGACCG250800.019.1961733
TTCGACG27000.019.1642786
AGAACGA267150.018.11553244
ATACGGA284400.017.00522833
TACGGAG284750.016.94480934
GACCGCG283900.016.9026135
AATACGG286950.016.86154432
CGTTACG22900.016.7004521
CCGCGTT290100.016.6723377
CGCGTTC293000.016.5222598
ACCGCGT294850.016.3511926
CGGTATA39900.015.84339120
CAATACG305750.015.823832531
AAGAACG308150.015.7724143
TCAGACC313650.015.7225742
GCGTTCT316950.015.3802349
ACGGAGA317550.015.29378435
CTCAGAC358150.015.0113241
Produced by FastQC (version 0.11.5)