FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48293325
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT864550.17902059963773462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC182950.022.8663464
CAGACCG188100.022.3719083
GAACGAT189000.021.90629445
TTCGACG23050.021.7711546
GACCGCG226150.018.4783345
AGAACGA232500.017.85608744
CCGCGTT235300.017.8357687
TCAGACC242850.017.7722262
ATACGGA234600.017.60768533
AATACGG235500.017.5308232
CGCGTTC239450.017.525278
ACCGCGT240450.017.4636256
TACGGAG235900.017.38715234
CTCAGAC283900.017.149091
CGTTACG16200.017.0815421
GCGTTCT250150.016.8100879
CAATACG253400.016.48739631
CGGTATA38850.016.44599520
AAGAACG255750.016.21510943
CGTACGC53050.015.6950915
Produced by FastQC (version 0.11.5)