FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48293325
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG18300.012.66824326
GGCATAG120150.012.56368135
AGCTTAG118850.012.52792845
ATAGGGG124550.012.33670538
GTCACTC190200.010.7789278
CATAGGG140550.010.72438737
GGTCACT188350.010.70373257
GGGAGGT208700.010.6049223
ACTCTCC197150.010.58286611
CACTCGA113750.010.3838845
ACACTCG117900.010.3797864
GCATAGG146500.010.30417936
TGGCATA148100.010.20777334
GAGCTTA149200.09.9953444
GGAGGTC237900.09.2479974
GAGGTCA240500.09.2425925
TAATCGG39550.09.2145014
ACTCGAG138500.09.1951086
TCACTCT239650.08.8558339
CACTCTC234200.08.85102510
Produced by FastQC (version 0.11.5)