FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52856240
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1330710.2517602462831257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT280750.024.11747645
AGACCGC300600.022.833574
CAGACCG312600.022.0289883
TTCGACG24050.021.6944946
GACCGCG336750.020.2220385
CCGCGTT343650.019.9207177
CGCGTTC344900.019.8093958
AGAACGA345250.019.68349344
ACCGCGT351850.019.4500646
TCAGACC374000.018.8456972
GCGTTCT371300.018.4252689
ATACGGA369400.018.17313633
AATACGG371200.018.13953832
AAGAACG375100.018.10509343
TACGGAG369550.018.05606334
CTCAGAC424550.017.8644051
CAATACG382300.017.7834631
ACGGAGA407250.016.48411635
CCCAATA418900.016.3318829
CCGTACG60150.016.2641724
Produced by FastQC (version 0.11.5)