FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49105836
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG125850.013.90924745
ATAGGGG134950.012.920650538
GGCATAG134000.012.86114335
GTTGTCG20800.012.76422926
GAGCTTA146800.011.92393944
GGGAGGT233500.011.3049683
CATAGGG155600.011.1047137
TGGCATA159900.010.84830634
GCATAGG163100.010.62165836
TCAGACG82900.010.5054812
GGAGGTC252950.010.1065244
ACTCTCC258350.09.75390211
ATAGCTC61650.09.59794539
AGGGATC141650.09.3892392
TAGCGTA6250.09.3594237
CGTGGCG134050.09.0655561
ACCCGGC71250.09.0647375
GGTCACT272450.08.9205137
GTCACTC279100.08.8369748
GCGTAGG73600.08.803802536
Produced by FastQC (version 0.11.5)