FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45433137
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT859460.18917029656129622No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC553210.12176354892685486No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGAT530440.11675178845783861No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG458930.10101217532040546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT237950.028.28955845
AGACCGC284100.023.965014
AGAACGA285200.023.74473244
CAGACCG290650.023.46373
TTCGGCG116250.023.39826444
GACCGCG302550.022.4589635
ACCGCGT307400.022.161056
CCGCGTT311550.021.8877587
CGCGTTC315400.021.61348
AATACGG319350.021.28954332
ATACGGG124600.021.19770633
TCAGACC335500.020.5961882
TTCGACG20750.020.491896
ATACGGA212050.019.71270633
TCGGCGC138700.019.69212245
CCGTACG141150.019.5429234
GCGTTCT350700.019.4122269
TACGGAG216300.019.38772234
CGTACGC142500.019.310415
AAGAACG354650.019.18368743
Produced by FastQC (version 0.11.5)