FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45433137
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG639050.1406572475944155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG185050.016.76060145
ACCCGGC105400.016.1163045
GGCATAG196250.015.65439635
TAGCGTA10650.015.62804637
GAGCTTA212550.014.66658544
TCACCCG120650.014.5652123
ATAGGGG214300.014.45171938
GGTCACT314400.014.4233577
TGGCATA215150.014.40466434
GTCACTC316350.014.3921468
ATAGCTC115750.013.814960539
ACTCTCC332150.013.70807311
ATAGCGT12650.013.69060936
AGGTCAC343550.013.5338196
GGGAGGT380300.013.2944343
GAGGTCA358550.013.0518685
GGAGGTC366700.012.7564124
CATAGGG245150.012.74362337
TAGCTCT126900.012.53005740
CCGGACA144600.012.37757812
Produced by FastQC (version 0.11.5)