FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58943451
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG619740.10514145159230667No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA603660.10241341315424507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACG146500.013.4602062
AGCTTAG207050.013.17291145
GCGTCGT31500.012.50097611
GGCATAG215200.012.34579435
GGGAGGT310250.011.826533
TAGCGTA12750.011.82310737
GTCACTC288700.011.5278158
GTTGTCG30750.011.48502626
ATAGGGG239100.011.48068838
GGTCACT290250.011.3808977
GAGCTTA239000.011.37426744
TGGCATA239400.011.17294334
ACTCTCC316500.010.81364111
GAGGTCA327950.010.8104875
CAGACGT181550.010.7622993
CATAGGG254800.010.57850837
ACCCGGC91300.010.4043275
TCGTGTA38750.010.3941314
CGTGGCG281550.010.3306311
TGCCCGT258900.010.31746644
Produced by FastQC (version 0.11.5)