FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48685035
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG132650.014.53882445
ATAGGGG140200.014.00975238
GGCATAG142350.013.82788935
GTTGTCG22300.012.70968826
CATAGGG157100.012.53007637
TGGCATA164350.012.195621534
GAGCTTA161150.012.06532944
GTCACTC229350.011.9806338
GGTCACT228750.011.9515937
GCATAGG168500.011.62868836
GGGAGGT255650.011.5530553
ACTCTCC241850.011.52890511
GAGGTCA268900.010.673415
GGAGGTC272750.010.4817114
AGGTCAC277450.010.4134256
TAGGGGA270600.09.96916439
ATAGCTC66050.09.84447139
TCACTCT288350.09.693149
TGTCGTT31850.09.53480528
TAGCTCT73700.09.31166540
Produced by FastQC (version 0.11.5)