FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44339048
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1549660.34950231678406807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC288900.025.9093284
CAGACCG294950.025.477043
ACCGCGT297800.025.0896766
GACCGCG298150.025.0224935
GAACGAT297850.024.86646545
CCGCGTT308900.024.1881057
CGCGTTC310550.024.0596458
TCAGACC339200.022.4055272
AGAACGA338050.021.9825744
GCGTTCT346500.021.6218519
CTCAGAC380450.021.3468631
AATACGG356250.020.6765532
TACGGAG357250.020.51784134
ATACGGA362450.020.35385133
AAGAACG375250.019.8213443
CAATACG374250.019.7304731
ACGGAGA380600.019.30044235
CGGAGAG392300.018.71335236
CCTCTCA408550.018.57043619
CGTTCTC411400.018.41330110
Produced by FastQC (version 0.11.5)