FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46669892
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG129650.012.49531445
ATAGGGG142150.011.65745738
GGCATAG141950.011.32094535
GGGAGGT230250.010.9250553
GTCACTC227950.010.2649638
GGTCACT225250.010.2589337
GAGCTTA160550.010.1246144
CATAGGG163100.010.06928837
TGGCATA160500.010.04596534
GGAGGTC248900.09.8898774
ACTCTCC241850.09.88138911
GTTGTCG21100.09.82849926
TCAGACG83050.09.8168512
GCATAGG170500.09.6968936
GAGGTCA270600.09.1957155
CGTGGCG118900.09.0760951
CACTCTC267600.08.90531710
TAGGCTA77050.08.8034810
TCTATAG235100.08.7794663
TCACTCT277350.08.7781249
Produced by FastQC (version 0.11.5)