FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49908431
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1345100.26951358178340645No Hit
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1194630.23936436711464643No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG794020.15909536406784658No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG619460.12411930962125417No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA537480.10769322722247068No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT515410.10327112867964132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCT455050.022.9063788
TTCACGC466100.022.4212387
AGACCGC309400.022.124654
CAGACCG317100.021.6513183
ACGCTCA496350.021.18628510
GACCGCG320650.021.1800335
ACCGCGT322950.021.048386
CCGCGTT330400.020.6146187
CGCGTTC330700.020.5415048
CACGCTC539750.019.9661889
AACGCTA521600.019.70777923
ACGCTAC526100.019.5264424
CGCTACC537250.019.11711925
AAAACGC542650.018.99303221
TCAGACC374500.018.9095272
GAAAACG546950.018.78613920
GCGTTCT365300.018.6760239
TACGCGT18250.018.51625644
GAACGAT371250.018.38051245
AAACGCT561250.018.36760722
Produced by FastQC (version 0.11.5)