FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49908431
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1109860.22237926093088362No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG656240.13148880596947637No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA631320.1264956616247864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT525550.015.6990083
GGAGGTC525950.015.400594
GAGGTCA531600.015.2055255
AGGTCAC539050.014.9094886
GTTGTCG35400.014.43359326
GGTCACT554350.014.2454387
TGGGAGG424150.013.7903872
AGGGATC219500.013.400742
GTCACTC589050.013.3640418
CTGGGAG475400.013.1551961
TCACTCT610050.013.1356969
ACTCTCC606500.012.96892711
CACTCTC610250.012.79261110
AGCTTAG456100.012.34129845
ACCCGGC76450.012.0585155
CAGGGAT268050.011.9635271
TGTCGTT43000.011.93530628
GGCATAG473000.011.88789135
TCAGACG118250.011.7718582
ATAGGGG490500.011.61965838
Produced by FastQC (version 0.11.5)