FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48932383
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC79700.013.1561235
ATAGCTC73300.012.95116839
TAGCTCT84050.011.56244340
CACCCGG95350.011.4915224
CGGCCCG103150.010.7113498
TATCCTA72700.010.646935
TGATAGC95150.010.63860537
CGGACAG95800.010.57031219
TCAGACG82250.010.5625662
TAGCGTA4950.010.45210137
TTGATAG100550.010.29082236
ACACGGA97650.010.23190216
AGCTTAG85450.010.18947445
GATAGCT97400.010.06947238
TAGGCTA72250.010.02942810
GTTGTCG19400.09.97320926
ATAGGGG89050.09.95271338
GGCATAG85700.09.92187435
CCGGACA112250.09.82389712
TCACCCG122100.09.6405323
Produced by FastQC (version 0.11.5)