FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42885992
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT720220.16793828623574802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT184850.024.98928345
AGACCGC194450.024.7847274
CAGACCG199500.024.292683
AGAACGA215700.021.33154744
GCGTTCT238700.020.4446549
ATACGGA234250.020.0358333
TACGGAG238750.019.59222434
AATACGG240500.019.55256832
CTCAGAC277050.019.2659241
GACCGCG257250.019.0403825
CAATACG254750.018.68848631
TCAGACC270200.018.4359252
ACCGCGT268200.018.321736
ACGGAGA258000.018.17400435
AAGAACG255400.018.07711443
CCGCGTT274450.017.9126917
CCCAATA266900.017.82087529
CGCGTTC276450.017.8075168
CGGAGAG275600.017.07054736
TTCGGCG80050.016.75190244
Produced by FastQC (version 0.11.5)