FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42885992
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA5900.014.4922637
AGCTTAG116600.014.45319845
ACCCGGC62050.013.6416455
GGCATAG122800.013.3752135
ATAGGGG132350.012.71688138
ATAGCTC63200.012.5678439
GAGCTTA136500.012.21349244
GGGAGGT209650.012.1461083
GTTGTCG13250.011.88495226
TCAGACG74700.011.6643292
CATAGGG147400.011.2505737
TAGCTCT71400.011.06143340
TCACCCG87300.010.8834753
ACTCTCC223550.010.86882511
TGGCATA154700.010.76260834
GGTCACT221900.010.6755527
GCATAGG156400.010.56039336
GTCACTC236950.010.3485858
TAGGCTA59600.010.19179110
ATAGCGT8350.09.97093736
Produced by FastQC (version 0.11.5)