FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43631678
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG565190.12953661786741277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG187350.014.55961945
GGGAGGT287600.014.0107693
ATAGGGG208050.013.27098838
GGCATAG204750.013.22102635
GGAGGTC302600.013.1747864
GGTCACT306850.012.6250017
GAGCTTA217700.012.48845244
GAGGTCA328600.012.235055
GTCACTC320150.012.1988988
ACTCTCC326700.012.16765311
TGGCATA230800.011.806634
TCACTCT351650.011.361929
GTTGTCG20300.011.30524126
CACTCTC349100.011.20004710
CATAGGG252500.010.8813137
TGGGAGG277750.010.3764862
GCATAGG270750.010.18107236
AGGTCAC401500.09.9569486
GCTCTGT404750.09.74988522
AGGGGAG407600.09.67332240
Produced by FastQC (version 0.11.5)