FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50480297
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG24350.011.72988226
AGCTTAG136350.011.70888345
ATAGGGG135750.011.49978938
GGCATAG137600.011.19812635
GGGAGGT220250.010.9710463
GTCACTC214400.010.3967798
GGTCACT213000.010.2747897
GAGCTTA161550.09.88254544
GGAGGTC247950.09.6351634
CATAGGG162800.09.63074337
ACTCTCC234200.09.581202511
GCATAGG170350.09.17749336
TGGCATA171950.09.06611234
TCACTCT270550.08.4716919
AGGTCAC278800.08.455456
ACCCGGC63500.08.4390795
CACTCTC262150.08.43201810
GAGGTCA279300.08.4001815
ATATAGC55500.08.27599056
TGTCGTT34950.08.23695628
Produced by FastQC (version 0.11.5)