FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45792844
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG959640.20956112706168678No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT643150.14044770837993814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG24850.022.1823926
GCCGGCT257450.019.782191
TTCACGC271050.018.8013277
CCGGCTT302000.018.5372312
TCACGCT277450.018.4001088
CGGTATA40800.018.0876820
GTCCGTT36700.017.3004631
TCGACGA31650.017.274377
CGTTACG13100.016.65990421
AGACCGC184900.016.5866414
CGGCTTC339100.016.5422293
ACGCTCA321250.016.2975910
AACGCTA305200.016.13747823
CAGACCG192400.016.1154963
ACGCTAC307850.016.0058924
CGCTACC314500.015.67472625
GTTCGAC35200.015.6611255
CACGCTC332550.015.5476289
AAAACGC323650.015.32173721
CGACGAG35700.015.188668
Produced by FastQC (version 0.11.5)