FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45792844
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA661620.14448108966545078No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG616610.13465204301353284No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG546010.11923478698986244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG209700.014.70382845
GGGAGGT311300.014.3596733
GGAGGTC333500.013.3098074
GGCATAG237900.012.90071235
ATAGGGG245400.012.645061538
GAGCTTA250800.012.30320344
TGGCATA254600.012.09920534
GTTGTCG21700.011.5083326
TGGGAGG299500.010.8767112
GGTCACT400700.010.8422197
GTCACTC410000.010.7606048
CATAGGG294450.010.54631537
ACTCTCC418700.010.49997411
GCATAGG303400.010.25696536
TATCCTA95000.010.1594225
TCTCCCC432400.010.13602313
CTGGGAG353500.010.0148781
GCTCTGT442700.09.92444422
AGCATAG125200.09.92345
TGTCCAA448300.09.8213526
Produced by FastQC (version 0.11.5)