FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50952080
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1129980.22177308561299167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG38200.026.5056746
GAACGAT306950.025.61126145
AGACCGC352150.022.2799384
TCGACGA45550.022.2775177
CAGACCG360100.021.8758143
GACCGCG360550.021.7296645
ACCGCGT368850.021.2711966
CCGCGTT381200.020.5874377
CGCGTTC384200.020.4504458
AGAACGA388050.020.27026444
CGGTATA62150.019.98355520
GTTCGAC49850.019.72455
TCAGACC412050.019.4345932
CGACGAG51250.019.3611288
GCGTTCT409900.019.1851229
TACGGAG418000.018.71019634
AATACGG419950.018.65010532
ATACGGA420150.018.61980633
AAGAACG425850.018.42873443
CTCAGAC468700.018.3694271
Produced by FastQC (version 0.11.5)