FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50952080
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG609320.11958687456920306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG37300.015.68396226
GGGAGGT327350.013.8534713
AGCTTAG228700.013.74692445
ATAGGGG246950.012.9136638
GGCATAG243250.012.81295235
GGAGGTC350300.012.7019254
GAGGTCA364100.012.3873985
GGTCACT353900.012.3565557
ATATAGC57300.012.2091836
GAGCTTA260600.012.18555744
GTCACTC369600.011.9594798
TGGCATA269500.011.69836734
ACTCTCC382350.011.63166111
TGTCGTT53850.011.53337628
AGGTCAC392050.011.4926546
TCACTCT411800.010.9797999
CACTCTC408500.010.92566610
GCTAAGT59100.010.92502914
TGGGAGG315800.010.7344922
GTCGTTC56800.010.61724529
Produced by FastQC (version 0.11.5)