FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44729801
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1448890.3239205110704606No Hit
CTCGCACCGAGACTCAAGGCCGTCTCCACAACTCCAACCAGTGCAAATGAC450880.10080080615605691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT251850.026.6283745
ACCGCGT291350.023.0876796
GACCGCG291950.023.0787565
AGACCGC294150.022.929094
CAGACCG300450.022.5531313
AGAACGA301100.022.33262444
CGCGTTC303900.022.03068
CCGCGTT306050.021.9199397
TCAGACC341950.020.0002332
AAGAACG337400.019.96327443
AATACGG345050.019.50676332
ATACGGA349250.019.30258233
TACGGAG354400.019.00380334
GCGTTCT354850.018.9815249
CTCAGAC392150.018.3772131
CAATACG377000.017.98502331
ACGGAGA379650.017.71048735
CGGAGAG391350.017.21558236
CCCAATA394950.017.19068729
CACTCCC411550.016.64934224
Produced by FastQC (version 0.11.5)