FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44729801
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG824900.184418437274067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG226600.017.8232645
ATAGGGG246000.016.54569838
GGCATAG252700.016.01797335
GGGAGGT360850.015.937393
GTCACTC365050.015.3348548
GGAGGTC370950.015.297234
GAGCTTA265100.015.2942344
AGGTCAC373500.015.2349246
TGGCATA272750.014.8817334
GAGGTCA392600.014.60839755
ACTCTCC386700.014.56943611
CATAGGG297000.013.8939137
TCACTCT414700.013.8135869
GGTCACT412550.013.4874287
CACTCTC421100.013.43802110
TGGGAGG323250.013.2598772
GCATAGG315200.013.02741636
GTTGTCG25750.012.49516226
CTGGGAG386350.012.1017371
TCTCCCC474300.011.9402313
Produced by FastQC (version 0.11.5)