FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48961701
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG22500.018.197216
CGTTACG19150.017.97668321
GTCCGTT40400.017.0472641
CCCGTAT35150.014.2788811
TCGACGA29450.013.902827
AACGCTA137800.013.8631323
ACGCTAC138500.013.85957824
AGACCGC68700.013.5896464
ACGTTCG12550.013.4441536
GCCGGCT160550.013.4017721
CGACGAG30850.013.2718978
CCGGCTT180850.013.1857282
CCCCCCT39100.013.0666341
CGCTACC146900.013.0526525
ACCGCGT69750.012.93356
CTCGGTT87450.012.9198811
GACCGCG70150.012.8918225
CAGACCG75400.012.769953
TTCACGC164400.012.6303877
CGGTATA38350.012.4362920
Produced by FastQC (version 0.11.5)