FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46881878
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG129550.011.17054338
AGCTTAG129050.011.12847645
GGCATAG127500.011.04852135
GGGAGGT202000.011.0369663
GGAGGTC208350.010.3013664
TCAGACG84800.010.0281772
TGGCATA145900.09.685863534
GAGCTTA147900.09.66448244
CATAGGG151700.09.58323137
GGTCACT216600.09.5153787
GTTGTCG19600.09.06701226
GCATAGG159200.09.03258736
ACTCTCC237900.09.02287311
ATAACGC27750.08.92178539
CGTAGGG69400.08.85254737
GCGTAGG70050.08.77018836
CAGACGT98200.08.7285263
CGTGGCG122950.08.6913351
ACCCGGC70900.08.6632515
GGCGTAG71450.08.62955335
Produced by FastQC (version 0.11.5)