FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43458078
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT917150.21104246717951955No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG630880.1451697886869272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC206300.022.0303544
GAACGAT209700.021.75899545
CAGACCG210250.021.6164653
GACCGCG216450.020.9141255
ACCGCGT221200.020.4548476
CCGCGTT228950.019.8115847
AACGCTA180950.019.69615223
CGCGTTC231900.019.462548
ACGCTAC183100.019.4527924
CGCTACC191050.018.58447325
TCACGCT200100.018.4964378
AGAACGA247050.018.4784944
AAAACGC198250.018.10165621
TTCGGCG73100.018.09794644
GAAAACG202150.017.75238820
ACGCTCA215700.017.555110
GCGTTCT259850.017.5163029
TTCACGC215350.017.301547
GCCGGCT190400.017.2199421
AATACGG263100.017.19776532
Produced by FastQC (version 0.11.5)