FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50903186
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1173940.230622106836299No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA672540.13212139609493204No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG606170.11908291948562906No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG516170.10140229729431868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT503400.016.8014953
AGGTCAC536300.015.597836
GGAGGTC538700.015.5918644
GGTCACT536250.015.3835547
GAGGTCA552600.015.27584655
GTCACTC550950.014.9246138
TGGGAGG412100.014.7619252
ACTCTCC576050.014.31777211
TCACTCT594850.013.9162789
CACTCTC596650.013.77068810
CTGGGAG486250.013.3942161
AGCTTAG442000.013.23349145
GGCATAG462800.012.66300235
ATAGGGG465500.012.60904938
CACCCGG145550.012.2683641
TGGCATA482450.012.14915534
GAGCTTA486800.012.00516144
AGGCTCT499300.011.99936520
TATCCTA125900.011.9666055
TCTCCCC706100.011.78318913
Produced by FastQC (version 0.11.5)