FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43965829
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG760040.17287061731509715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA188850.021.15584423
ACGCTAC191100.020.97762724
CGCTACC197050.020.33280625
TCACGCT202250.020.3324688
TTCACGC203250.020.3320667
CCGGCTT224000.020.0526832
AAAACGC203750.019.58666221
ACGCTCA216350.019.34081610
GAAAACG210400.019.0316620
AAACGCT216050.018.54445322
GCCGGCT224700.017.8641951
AGACCGC74500.016.9494674
CGGCTTC279750.016.7169763
CACGCTC253500.016.3732419
GCACTAC83300.016.2037725
CCCAGAT188450.015.6793891
GACCGCG80550.015.5319965
CCCGTAT36750.015.5162131
CAGACCG83150.015.2600473
GTTCCTA62250.014.96165815
Produced by FastQC (version 0.11.5)