FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48400410
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA948700.19601073627268858No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG909210.18785171447927818No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG629780.13011873246528283No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG581510.12014567645191436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG38050.015.43135726
GGGAGGT317950.014.2506043
AGCTTAG231350.013.699409545
ATATAGC51150.013.3723076
GGAGGTC350300.012.7617464
GGTCACT350950.012.4504387
ATAGGGG257450.012.43879738
GGCATAG253750.012.41516435
GTCACTC350700.012.3374158
TGTCGTT49250.012.05913128
GAGCTTA265800.011.8984444
GCTAAGT52650.011.836114
GTCGTTC52750.011.55753229
GCGTCGT26300.011.54924911
TGGGAGG295900.011.4653472
GAGGTCA400900.011.3981845
ACTCTCC395400.010.98237611
TGGCATA291800.010.87332834
TCACTCT404950.010.856859
AGGGATC240850.010.8446722
Produced by FastQC (version 0.11.5)