FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44739465
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1140150.25484211758008285No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG626240.13997485218028424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC263900.021.1594754
ACCGCGT264600.020.9926596
GAACGAT266750.020.63804845
GACCGCG269500.020.6279475
CAGACCG273600.020.5326483
CCGCGTT274750.020.1598137
CGCGTTC277850.019.9510828
TTCGACG18250.018.7377266
TCAGACC308300.018.5135382
GCGTTCT317300.017.4563799
CTCAGAC358850.017.1743181
AGAACGA322700.017.08757844
CGTTACG13050.016.89731621
CCGGCTT242100.016.1602212
GCCGGCT206100.016.1091651
TCACGCT221550.016.0747918
GTCCGTT30700.016.0570131
AATACGG349800.015.6897532
TACGGAG349200.015.66415934
ATACGGA353000.015.62935733
Produced by FastQC (version 0.11.5)