FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44739465
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG665490.14874786723533684No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG506880.11329594576063885No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA463070.10350369634505018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG29500.016.9321726
AGCTTAG214650.015.88050845
GGGAGGT322550.015.0534513
GGAGGTC329200.014.4623064
GGCATAG234550.014.43719335
ATAGGGG244100.014.02907638
GAGCTTA250750.013.64804644
TGGCATA254250.013.43360334
ACTCTCC351300.013.14261711
ATATAGC44550.013.1312736
GGTCACT354850.013.0744987
TGTCGTT40000.012.59996128
GTCACTC379700.012.25437458
TGGGAGG295000.012.2263432
CTGGGAG328600.011.9279461
CATAGGG287050.011.91427837
CACTCTC384450.011.87476110
GCTAAGT45100.011.82376914
TCACTCT400750.011.8184439
GAGGTCA416550.011.6348365
Produced by FastQC (version 0.11.5)