FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57706657
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1174600.20354670692499136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC252900.022.6463054
CAGACCG263000.021.9819773
GAACGAT265100.021.49014745
TTCGACG25400.020.4623996
GACCGCG295900.019.2793065
ACCGCGT315800.018.1252176
AGAACGA323350.017.71615244
CCGCGTT326200.017.56057
GCGTTCT330050.017.4238119
CGCGTTC330250.017.3996338
TCAGACC343400.017.1498952
TACGGAG333600.017.08393134
ATACGGA334700.017.05468833
CTCAGAC374650.017.0055081
AATACGG335250.016.9931532
CCCGTAT34700.016.8683591
AAGAACG356200.016.10113343
CAATACG356800.016.03616531
CCGTACG69950.015.6674984
ACGGAGA366450.015.54019335
Produced by FastQC (version 0.11.5)