FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57706657
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG585680.10149262328607947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG206250.014.49948945
GGGAGGT345900.013.422763
GGCATAG226000.013.32254235
ATAGGGG228100.013.30818838
GTCACTC330450.012.8533828
GGTCACT332350.012.8196587
GAGCTTA244800.012.32651144
GTTGTCG32550.012.30551426
AGGTCAC369300.012.0368756
GGAGGTC371400.012.0048754
ACTCTCC352150.011.97902911
TGGCATA261500.011.61720234
TCACTCT370950.011.6140899
GAGGTCA388250.011.4898395
CACTCTC381150.011.12024810
CATAGGG275050.011.07768637
GCATAGG289850.010.55081436
ACCCGGC79100.010.5227185
TGGGAGG320900.010.3185372
ATATAGC54150.010.2613536
Produced by FastQC (version 0.11.5)