FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47223558
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG28850.014.97359926
GGGAGGT242250.012.5972893
AGCTTAG161800.012.58486845
ATAGGGG167900.012.20753338
GGCATAG167700.012.16865935
GTCGTTC37500.011.7596729
TGTCGTT36600.011.74142528
GGAGGTC263850.011.3513484
GCTAAGT39850.011.00934414
GAGCTTA187350.010.94034144
TGGCATA195550.010.65424334
GGTCACT271250.010.5432317
ATATAGC49850.010.2463626
CATAGGG203550.010.2354337
GTCACTC289300.010.1102968
ACTCTCC292650.010.02500711
GCATAGG214350.09.64626436
TAATCGG33850.09.5726094
TTGTCGT53250.09.337687527
TCGTTCC47050.09.32491530
Produced by FastQC (version 0.11.5)