FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47237715
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG531330.11248003846079345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG168450.015.26742145
GGCATAG180600.014.28978135
ATAGGGG187150.013.84976938
GAGCTTA191400.013.38964444
GGGAGGT280250.013.2954733
TGGCATA199950.012.88439434
GGAGGTC296350.012.2087214
GTCACTC294850.012.1637398
CATAGGG216600.011.95629937
ACTCTCC305900.011.51846111
GCATAGG227850.011.4647136
GTTGTCG18850.010.38457426
TGGGAGG273950.010.1434272
GGTCACT355600.09.9844837
TAGGGGA353150.09.86898639
TCACTCT376250.09.7893419
CACTCTC369150.09.660710
GCTCTGT370900.09.63328622
TGTCCAA381350.09.48730926
TCTCCCC382100.09.3803913
Produced by FastQC (version 0.11.5)