FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43591304
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT505110.1158740284530144No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC454260.10420885780338207No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGAT444850.10205017037343043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT157600.028.69017845
TTCGGCG86350.025.04854844
AGAACGA191600.023.6701544
AGACCGC199300.022.6879884
CAGACCG205350.022.1181553
AATACGG206350.021.82304232
GACCGCG207750.021.765185
ACCGCGT212050.021.398116
TCGGCGC102600.021.2342845
CCGCGTT214550.021.1383827
CGCGTTC215950.021.0221778
AAGAACG215900.021.01645343
ATACGGG105700.020.54676633
ATACGGA116950.020.26372333
CAATACG225050.020.12289431
TACGGAG118850.019.88390534
CCGTACG110250.019.8741074
CGTACGC111950.019.5120285
TACGGGG114650.019.04185334
ATTCGGC117500.018.88689643
Produced by FastQC (version 0.11.5)