FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43591304
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG82200.010.53980338
GGCATAG84500.010.25267435
CATAGGG86350.010.05924937
AGCTTAG89400.09.8413145
TAGGCTA52100.09.45735610
CCGGTAA18200.09.3951912
TGCCCGT137950.09.3303344
GCATAGG95000.09.28545236
TGGCATA102850.08.68459534
TCAGACG80000.08.6621182
ACTCTCC159850.08.642096511
CGTAGGG47250.08.57257537
TATCCTA63700.08.5121845
ACGCGCG17350.08.42933320
GCGTAGG48250.08.34826736
GGCGTAG48100.08.327373535
GGGAGGT181550.08.1544183
ATCCTAG82850.08.0654256
ATAGCTC52450.08.02305339
GAGCTTA110800.08.02195144
Produced by FastQC (version 0.11.5)