FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48493905
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT645450.1330992008170924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGCG65350.019.4803744
AGACCGC160700.019.218914
GAACGAT158650.018.7752545
GACCGCG169550.018.3218785
CAGACCG169400.018.3115443
ACCGCGT173350.017.9964126
CCGCGTT177450.017.5552527
CCGTACG74700.017.5257434
CGTACGC75600.017.227845
GTCCGTT33700.017.1635681
CGCGTTC182800.017.0537688
TCGGCGC75700.016.66811645
AGAACGA188350.015.91037444
TACGCCA86450.015.2723697
CGCCGCG83750.015.22824828
CGTTACG18300.015.11823921
CCCGTAT32750.015.0500221
CGGGGCG85900.014.90418633
CGCCCCG88950.014.76772723
TCCGTAC91250.014.7661763
Produced by FastQC (version 0.11.5)