FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51617510
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT559170.1083295184134221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC132150.021.2287654
GACCGCG136150.020.6216015
ACCGCGT138450.020.2140276
CAGACCG142050.019.9076273
CCGCGTT151450.018.7156667
CGCGTTC153350.018.4539228
GTCCGTT37500.015.923241
GAACGAT177100.015.75245945
TCAGACC199500.014.47930052
GCACTAC73800.014.41950825
CTCAGAC239900.014.1640641
GCGTTCT200500.014.1591389
CCCGGAT117850.013.995821
CCCGTAT39650.013.9800511
TACGGAG204850.013.58975134
AATACGG205050.013.56515332
CGTTACG17300.013.5239221
ATACGGA207600.013.35553133
GCCGGCT157600.013.3109881
AGAACGA210300.013.23401544
Produced by FastQC (version 0.11.5)