FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51617510
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG25950.011.79169426
AGCTTAG137900.011.51891945
ATAGGGG142350.011.31698138
GGCATAG143650.010.729335
GGGAGGT226800.010.4164173
GAGCTTA164950.09.84818444
GGAGGTC234000.09.7401514
CATAGGG169400.09.31064337
TAGGCTA70600.09.01894110
TGGCATA173850.08.94313534
TCAGACG76450.08.7702282
GCATAGG179400.08.71663436
GGTCACT258450.08.4705217
ACTCTCC267050.08.46734511
CCTAGGC82450.08.2957718
ATCCTAG95950.08.2306846
TATCCTA90100.08.1906875
GCGTTCG16000.08.15603915
GTCACTC282050.07.9851268
GAGGTCA306850.07.9849665
Produced by FastQC (version 0.11.5)