FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49373266
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG511770.10365326045070626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC163050.021.558024
CAGACCG169150.020.9137883
GACCGCG173200.020.0338925
ACCGCGT173450.019.9788276
CCGCGTT177500.019.5351357
CGCGTTC179100.019.335398
ATACGGG96050.018.03611633
TACGGGG95400.017.99386834
TCAGACC214800.016.9613822
CGCTACC186200.016.95236425
AACGCTA206650.016.95179223
ACGCTAC210550.016.71235824
GCCGGCT208150.016.6692471
CCGGCTT227500.016.6376972
TCACGCT220950.016.6303778
TTCACGC222450.016.558787
GCGTTCT218800.015.8971839
GAAAACG213000.015.72811420
AAAACGC225850.015.6501521
ACGCTCA243050.015.43076510
Produced by FastQC (version 0.11.5)