FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49373266
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG155500.012.77566238
GGCATAG159600.012.39242435
AGCTTAG162350.012.30285445
GGGAGGT255200.012.14199353
GGAGGTC260600.011.5973114
GAGCTTA185050.010.90348444
TGGCATA186100.010.67614834
CATAGGG204900.09.78455937
GTCACTC304250.09.6226378
ACTCTCC308650.09.57443711
ATAACGC54850.09.5565539
GGTCACT315300.09.3279797
GTTGTCG20550.09.30955726
GCATAGG215700.09.25299136
TAACGCC58400.09.1280640
AGGGATC142000.09.0728952
CGTAACG13500.08.8307242
CACTCTC337700.08.81056510
GCTCTGT338700.08.70701522
TAGGGGA342250.08.46631139
Produced by FastQC (version 0.11.5)