FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45009242
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG124500.014.1541845
ATAGGGG136500.013.138838
GGCATAG137150.012.94491435
GGGAGGT217100.011.8861563
GGTCACT210400.011.6012037
CATAGGG155150.011.57378437
GAGCTTA157100.011.4030144
GTCACTC220600.011.2483438
ACTCTCC224500.011.0930711
GCATAGG163000.011.04396836
GTTGTCG19500.010.96125126
TGGCATA164900.010.83469934
TATCCTA87750.010.4859035
AGGTCAC254500.010.0859536
TAGGCTA86850.010.0244310
CACTCTC252050.09.88051210
TCACTCT261500.09.747139
ATCCTAG103800.09.7095636
GGAGGTC274750.09.5149454
TGTATCC103100.09.4704133
Produced by FastQC (version 0.11.5)