FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50337385
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1521140.3021889198256922No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1190880.23657963161971962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC347250.022.9034924
CAGACCG357250.022.2938773
GAACGAT366550.021.58620645
GCCGGCT286650.021.455621
GACCGCG375750.021.244145
CCGGCTT335200.021.0018022
ACCGCGT388200.020.5866
GCGTTCT401500.020.0441259
CCGCGTT402550.020.0421437
TCACGCT345150.019.97268
CGCGTTC404850.019.9338388
TTCACGC353850.019.5959857
TCAGACC418900.019.4371782
TTCGACG16000.019.1237686
CTCAGAC470050.018.7191491
AGAACGA427700.018.5315144
ACGCTCA389900.018.28074310
CGGCTTC400750.018.1504213
AACGCTA374250.017.981223
ACGCTAC375950.017.94176524
Produced by FastQC (version 0.11.5)