FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50337385
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG841530.16717793345840273No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA679820.1350527048633933No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG638120.1267686034942022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT396250.015.640443
AGCTTAG269300.015.251471545
GGAGGTC421100.014.3645154
GGCATAG296250.013.88199935
ATAGGGG301200.013.79743738
GTTGTCG23000.013.39997626
GAGCTTA310250.013.31477844
TGGCATA322650.012.78681934
TGGGAGG343000.012.7485072
ACTCTCC501450.012.06805711
CTGGGAG398700.012.0461031
GGTCACT497950.012.0033327
GTCACTC525000.011.5394528
TCAGACG108000.011.5234372
CACTCTC533450.011.3736410
TCTCCCC551900.010.95619713
AGGGATC202250.010.9270682
GCTCTGT553650.010.7517422
CATAGGG391550.010.67573637
CTCCCCA403400.010.66049514
Produced by FastQC (version 0.11.5)