FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57532412
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG605560.10525545148359154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG201850.014.56715345
GGCATAG210400.013.77922935
ATAGGGG215200.013.640985538
GGTCACT305050.013.4589667
GTCACTC307050.013.3782568
GGGAGGT327700.013.2129693
TGGCATA232150.012.55607934
ACTCTCC333700.012.3933611
GAGCTTA242850.012.255383544
CATAGGG241850.012.17412737
GGAGGTC356000.011.8270054
GAGGTCA363000.011.8081585
GCATAGG253900.011.62223536
TCACTCT370950.011.2868329
TATCCTA128200.010.4714835
CACTCTC414200.010.36455310
TGCCCGT215700.010.19985244
TAGCGTA8100.09.99681737
TCCTAGG134200.09.9575817
GCTCTGT416550.09.948853522
Produced by FastQC (version 0.11.5)