FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52181648
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT871410.16699549236160574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT172100.023.10622845
TTCGACG22100.019.7320166
AGACCGC209800.019.0795974
CAGACCG219550.018.4271743
AGAACGA219700.018.1609244
ATACGGA241800.016.54211833
TACGGAG242700.016.47964534
AATACGG243100.016.4258832
AAGAACG246950.016.2502143
GACCGCG250800.015.9874395
ACCGCGT261450.015.4669436
CAATACG259550.015.39295231
CCGCGTT266000.015.2190017
CGCGTTC266700.015.2037298
CGGTATA40200.015.15247520
CGTTACG14700.014.98479321
ACGGAGA273300.014.75267635
CCGTACG78300.014.7419314
GTCCGTT37800.014.704581
TCAGACC280100.014.6606172
Produced by FastQC (version 0.11.5)