FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005201596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005201596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45329505
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT794500.1752721544168638No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG510110.11253376801710056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC171700.023.1269654
CAGACCG175550.022.7222923
GACCGCG178950.022.1774255
ACCGCGT187150.021.2898676
TCAGACC226150.018.136982
CCGCGTT223600.017.8193137
TTCGACG21150.017.2325236
CTCAGAC266550.016.995841
CGCGTTC236050.016.8794338
CGTTACG37900.016.8027621
TCGACGA24800.015.1498757
GCGTTCT271850.014.7641629
CCGTACG80700.013.7162644
CGTACGC81150.013.695655
ACGCTAC154750.013.05805824
CTACACG52250.012.96063745
TCCGTTA51600.012.95031219
TAATTCG72300.012.94491740
AACGCTA155600.012.9433123
CGCTACC159200.012.69305425
Produced by FastQC (version 0.11.5)